Synthesis of the vasoconstrictor peptide endothelin in kidney cells

FEBS Letters

Itoh, Yasuaki¹  Onda, Haruo¹  Yasuhara, Tadashi¹  Matsumoto, Hirokazu¹  Kosaka, Takuo¹  Suzuki, Nobuhiro¹  Fujino, Masahiko¹ 

[1] Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd, Wadai-7, Tsukuba-shi, Ibaraki 300-42, Japan

关键词: Preproendothelin;    Gene expression;    (COS-7 cell;    Kidney cell;    Human);    ET-1 (2;    3);    endothelin 1 (2;    3);   

DOI  :  10.1016/0014-5793(89)80011-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression plasmid containing human prepro-endothelin cDNA was constructed and introduced into COS-7 cells. Mature endothelin, consisting of 21 amino acid residues, was secreted into the culture medium of the transfected cells and was also synthesized by non-transfected COS-7 cells. Normal kidney cells derived from other species also synthesized and secreted endothelins. Partial characterization of endothelins produced by kidney cells suggested the existence of new types of endothelin. This is the first report of the vasoconstrictor peptide endothelin being synthesized in kidney cells.

【 授权许可】

Unknown