Isolation of cDNA clones encoding the human Sm B/B′auto‐immune antigen and specifically reacting with human anti‐Sm auto‐immune sera

FEBS Letters

Coles, B.¹  Latchman, D.S.²  Sharpe, N.G.²  Howarth, D.N.²  Williams, D.G.³ 

[1] Cancer Research Campaign Protein Sequencing Facility, Department of Biochemistry, University College and Middlesex School of Medicine, The Windeyer Building, Cleveland Street, London W1P 6DB, England;Medical Molecular Biology Unit, London W1P 6DB, England;Division of Clinical Immunology, Mathilda and Terence Kennedy Institute of Rheumatology, London W6 7DW, England

关键词: Protein;    SmB;    Protein B′;    Autoimmune antigen;    Systemic lupus erythematosus;    RNA splicing;    alternative;   

DOI  :  10.1016/0014-5793(89)80801-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone for the human SmB and B′ auto-immune antigens has been isolated by antibody screening of a cDNA expression library. The cDNA clone hybridises with two distinct mRNAs, one of which is expressed in a tissue-specific manner. A fusion protein expressed from the cDNA clone was recognised by a number of sera from systemic lupus erythematosus (SLE) patients containing anti-Sm antibodies but not by sera reactive with other auto-immune antigens. The potential use of this clone in a diagnostic assay for SLE and in elucidating the processes regulating the expression of SmB and B′ is discussed.

【 授权许可】

Unknown