A pseudosubstrate peptide inhibits protein kinase C‐mediated phosphorylation in permeabilized Rat‐1 cells

FEBS Letters

Alblas, Jacqueline¹  Ploegh, Hidde¹  van Overveld, Merian¹  Moolenaar, Wouter¹  Eichholtz, Thomas¹ 

[1] Department of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121,1066 CX Amsterdam, The Netherlands

关键词: Signal transduction;    Protein kinase C;    Protein;    80 kDa;    Pseudosubstrate peptide;    (Rat-1 cell);   

DOI  :  10.1016/0014-5793(90)80657-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digiton-in-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC₅₀ of 1 μM, while a control peptide had no effect. The results indicate that this semi-intact cell system can be used in combination with the inhibitory pseudosubstrate peptide to study the involvement of PKC in cellular processes.

【 授权许可】

Unknown