The C‐terminal, 23 kDa peptide of E. coli haemolysin 2001 contains all the information necessary for its secretion by the haemolysin (Hly) export machinery

FEBS Letters

Mackman, N.²  Nicaud, J.-M.¹  Gray, L.²  Holland, I.B.² 

[1] Department of Genetics,University of Leicester, University Road, Leicester LEI 7RH, England;Institut National Agronomique Paris, 16, rue Claude-Bernard, 75231 Paris Cedex 05, France

关键词: (E. coli) Hemolysin C-terminal secretion signal Expression vector Protein export;   

DOI  :  10.1016/0014-5793(86)80838-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this paper we show the construction of a plasmid pLG609 which carries the 3'-end of the haemolysin structural gene, hlyA under tac promoter control. Expression of pLG609 in an E. coli strain carrying the haemolysin export genes hlyB and hlyD led to the efficient secretion of the C-terminal, 23 kDa peptide of haemolysin. The discovery of a C-terminal topogenic sequence, which appears to be all that is required for secretion of the whole toxin, is so far quite unique in protein export.

【 授权许可】

Unknown