Detection of a proteolytic activity in the micrococcal nuclease used for preparation of messenger‐dependent reticulocyte lysates

FEBS Letters

Demangeat, Gérard¹  Greif, Charles¹  Fritsch, Christiane¹  Hemmer, Odile¹ 

[1] Laboratoire de Virologie, Institut de Biologie Moléculaire des Plantes (IBMP) 12, rue du Général Zimmer, 67000 Strasbourg, France

关键词: Tomato black ring virus;    Translation;    in vitro;    Proteolytic processing;    Micrococcal nuclease;   

DOI  :  10.1016/0014-5793(89)80755-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Some, but not all, commercial preparations of micrococcal nuclease used to remove endogenous mRNA from reticulocyte lysates were found to contain proteolytic activity. The protease(s) caused a time-dependent cleavage of the polyprotein primary translation products of genome RNA of several plant viruses, but did not affect the translation products of some other virus mRNA. The activity resulted in the production of smaller proteins and was inhibited by zinc ions. Thus the protease(s) mimicked virus-coded proteases and represents a potential artifact in studies of translation products of virus RNA.

【 授权许可】

Unknown