FEBS Letters | |
Interaction of antibodies to synthetic peptides of proNGF with in vitro synthesized NGF precursors | |
Dicou, Eleni1  | |
[1] INSERM U 298, CHRU, 49033 Angers Cedex, France | |
关键词: Anti-peptide serum; Nerve growth factor; prepro-; Translation; in vitro; Immunoprecipitation; Processing; | |
DOI : 10.1016/0014-5793(89)81094-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Sera raised against three synthetic peptides that reproduce sequences of the pro-nerve growth factor (proNGF) protein were tested in immunoprecipitation experiments using in vitro translation products of SP6-directed NGF mRNA in a rabbit reticulocyte lysate. The interaction of these antibodies with bacterially synthesized chimeric preproNGF was also examined. Digestion of the translation products by the γ-subunit generated the 22 and 18 kDa intermediates. A predominant 13 kDa intermediate was obtained after digestion of translation products in wheat germ extract. This is shown to be the N-terminal peptide by immunoprecipitation with an anti-peptide serum. These antibodies may be used to detect NGF precursor cleavage products in vivo.
【 授权许可】
Unknown
【 预 览 】
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