Interaction of antibodies to synthetic peptides of proNGF with in vitro synthesized NGF precursors

FEBS Letters

Dicou, Eleni¹ 

[1] INSERM U 298, CHRU, 49033 Angers Cedex, France

关键词: Anti-peptide serum;    Nerve growth factor;    prepro-;    Translation;    in vitro;    Immunoprecipitation;    Processing;   

DOI  :  10.1016/0014-5793(89)81094-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sera raised against three synthetic peptides that reproduce sequences of the pro-nerve growth factor (proNGF) protein were tested in immunoprecipitation experiments using in vitro translation products of SP6-directed NGF mRNA in a rabbit reticulocyte lysate. The interaction of these antibodies with bacterially synthesized chimeric preproNGF was also examined. Digestion of the translation products by the γ-subunit generated the 22 and 18 kDa intermediates. A predominant 13 kDa intermediate was obtained after digestion of translation products in wheat germ extract. This is shown to be the N-terminal peptide by immunoprecipitation with an anti-peptide serum. These antibodies may be used to detect NGF precursor cleavage products in vivo.

【 授权许可】

Unknown