Stimulation of phosphoinositide hydrolysis via class I antigen‐specific recognition in murine cardiac tissue

FEBS Letters

Sterin-Borda, Leonor¹  Cremaschi, Graciela A.¹ 

[1] Centro de Estudios Farmacológicos y de Principios Naturales, CONICET, Serrano 665, (1414) Buenos Aires, Argentina

关键词: Histocompatibility antigen;    Alloantibody;    Phosphoinositide turnover;    Phospholipase C;    Adrenergic receptor;    α-;    Muscarinic cholinergic receptor;   

DOI  :  10.1016/0014-5793(89)80646-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Induction of polyphosphoinositide hydrolysis in cardiac tissue by specific recognition of class I histocompatibility antigens was assayed. C3H (H-2^k) mice auricles were labelled with myo-³H]inositol precursor and inositol phosphate production in the presence or absence of anti-class I k products was measured. Anti-class I, but not anti-class II products specifically increased phosphoinositide turnover. This increment was partially blocked by muscarinic cholinergic and α-adrenergic blockers and even more so by the phospholipase C inhibitor NCDC. Alloantibodies specifically directed against class I antigens could then exert stimulation of phospholipase C-mediated phosphoinositide hydrolysis through the interaction with muscarinic cholinergic and/or α-adrenergic receptors. The induction of intracellular second messengers by class I antigens and hormone-receptor interactions is discussed.

【 授权许可】

Unknown