Immunoprecipitation of insulin receptors by antibodies against Class 1 antigens of the murine H‐2 major histocompatibility complex

FEBS Letters

Chvatchko, Yolande¹  Fehlmann, Max¹  Kiger, Nicole²  Obberghen, Emmanuel Van¹ 

[1] INSERM U 145, Faculté de Médecine, Chemin de Vallombrose, 06034 Nice Cédex, France;INSERM U 210, Faculté de Médecine, Chemin de Vallombrose, 06034 Nice Cédex, France

关键词: Insulin receptor;    Histocompatibility antigen;    Monoclonal antibody;    Immunoprecipitation;    Photoaffinity labeling;   

DOI  :  10.1016/0014-5793(83)80820-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin receptors from C57BL/6J mouse (H-2^b) liver membranes were specifically labeled with ¹²⁵I-photo-reactive insulin by UV irradiation. Membranes were solubilized and the capacity of various antibodies reacting with the major histocompatibility complex to immunoprecipitate insulin receptors was tested. About 5% of the labeled receptors were immunoprecipitated by a conventional mouse antiserum against H-2^b histocompatibility antigens and by a monoclonal antibody against Class 1 antigens of the H-2^b haplotype (K^b and D^b). No immunoprecipitation was obtained with a monoclonal antibody against Class 2 antigens of I-A^b or against Class 1 antigens of the H-2^k haplotype. Insulin receptors can thus be specifically immunoprecipitated by antibodies against class I histocompatibility antigens.

【 授权许可】

Unknown