Detection of immunoreactive endothelin in plasma of hemodialysis patients

FEBS Letters

Ohneda, Minoru¹  Mouri, Toraichi¹  Takahashi, Kazuhiro¹  Yoshinaga, Kaoru¹  Saito, Takao¹  Totsune, Kazuhito¹  Sone, Masahiko¹ 

[1] Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai 980, Japan

关键词: Endothelin;    Radioimmunoassay;    Hemodialysis;    Immunoreactivity;    (Human plasma);   

DOI  :  10.1016/0014-5793(89)80631-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C₁₈ cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C₁₈ cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.

【 授权许可】

Unknown