Presence of immunoreactive endothelin in human plasma

FEBS Letters

Ando, K.¹  Marumo, F.¹  Emori, T.¹  Shichiri, M.¹  Hirata, Y.¹ 

[1] Second Department of Internal Medicine, Tokyo Medical and Dental University, Yushima, Tokyo 113 Japan

关键词: Endothelin;    Radioimmunoassay;    Reverse-phase HPLC;    (Human plasma);   

DOI  :  10.1016/0014-5793(89)80213-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1–21) and another with more hydrophilicity than hET(1–21). These data indicate that ET is a circulating vasoconstrictor hormone in man.

【 授权许可】

Unknown