The identification and characterization of α‐N‐acetylated β‐endorphin in the human pituitary gland

FEBS Letters

Cheng, M.C.¹  Smith, A.I.¹  Funder, J.W.¹ 

[1] Medical Research Centre, Prince Henry's Hospital, St. Kilda Road, Melbourne, Victoria 3004, Australia

关键词: Acetylation;    β-Endorphin;    Human pituitary;    Reverse-phase HPLC;    Radioimmunoassay;   

DOI  :  10.1016/0014-5793(85)80750-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By using a radioimmunoassay specific for α-N-acetyl β-endorphin and its C-terminally shortened forms, we have established the presence of immunoreactive α-N-acetyl endorphin (irNacEP) in extracts of five postmortem human pituitary glands (2.27 ± 0.64 ng/gland). This immunoreactivity has been further characterized by subjecting these extracts to reverse-phase high-performance liquid chromatography (RP-HPLC). In all cases the major peaks of irNacEP co-migrated with synthetic human standard α-N-acetyl α-endorphin (NacαEP), α-N-acetyl γ-endorphin (NacγEP) and NacβEP. These studies thus represent the initial demonstration that α-N-acetylation of β-endorphin and its shorter molecular forms occurs in the human pituitary gland.

【 授权许可】

Unknown