Monomeric pituitary growth hormone and prolactin variants in man characterized by immunoperoxidase electrophoresis

FEBS Letters

Vilamala, Maria¹  Christophe, Jean²  Robyn, Claude¹  Meuris, Sylvain¹  Svoboda, Michal² 

[1]Human Reproduction Research Unit, Unviersité Libre de Bruxelles at the Hôpital Saint-Pierre, 322 Rue Haute, B-1000 Brussels, Belgium

[2]Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, 115 Boulevard de Waterloo, B-1000 Brussels, Belgium

关键词: Growth hormone;    Prolactin;    Human pituitary;    Pleomorphism;    Immunochemistry;    Electrophoresis;    GH;    growth hormone;    h;    human;    hPL;    human placental lactogen;    ICC;    immunocytochemistry;    IPE;    immunoperoxidase electrophoresis;    ME;    2-mercapthoethanol;    NRS;    normal rabbit serum;    NSS;    normal sheep serum;    NTC;    nitrocellulose;    PAGE;    polyacrylamide gel electrophoresis;    PRL;    prolactin;    sA-rGG;    sheep anti-rabbit gamma globulin;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(83)80885-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoperoxidase electrophoresis, combining SDS-ME-PAGE and the ‘double bridge’ immunoperoxidase staining was applied to crude human pituitary homogenates. With anti-hGH and anti-hPL sera, 4 hGH-related monomers were characterized: a M _r 22 000 peptide corresponding to hGH; a M _r 20 000 peptide corresponding to the known hGH variant and two unknown hGH variants (M _r 65 000 and M _r 75 000). With anti-ovine, rat and human PRL sera, 4 PRL-related monomers were immunostained: one comigrated with purified hPRL (M _r 25 000), and 3 were unknown (M _r 29 000; M _r 45 000; M _r 16 000).

【 授权许可】

Unknown