Azidocytidine is incorporated into RNA of 3T6 mouse fibroblasts

FEBS Letters

Åkerblom, Lennart¹ 

[1] Department of Biochemistry I, Medical Nobel Institute Karolinska Institute!, S-104 01 Stockholm, Sweden

关键词: 2'-Azido-2'-deoxycytidine;    Ribonucleotide reductase inhibitor;    Cs2SO4 density gradient;    Reverse-phase HPLC;    3T6 cell;   

DOI  :  10.1016/0014-5793(85)80151-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Earlier work has shown that azidocytidine inhibits the growth and DNA synthesis of 3T6 mouse fibroblasts by inactivation of the enzyme ribonucleotide reductase. RNA synthesis, as measured by incorporation of [³H]cytidine was not affected. Here I show that azidocytidine is incorporated into RNA, but not into DNA. Incorporation of the analogue into RNA may under special circumstances contribute to the biological effect of the nucleoside.

【 授权许可】

Unknown