Human and porcine immunoreactive gastric inhibitory polypeptides (IR‐GIP) are not identical

FEBS Letters

Bacarese-Hamilton, A.J.¹  Bloom, S.R.¹  Adrian, T.E.¹ 

[1] Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Ducane Road, London W12 OHS, England

关键词: Gastric inhibitory polypeptide;    Glucose-dependent insulinotropic peptide;    Immunoreactive GIP;    Molecular form;    Species difference;    Gel filtration;    Reverse-phase HPLC;   

DOI  :  10.1016/0014-5793(84)80220-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoreactive gastric inhibitory polypeptide (IR-GIP) from human and porcine intestine was quantified by radioimmunoassay and the molecular forms characterised by gel permeation and reverse-phase high pressure liquid chromatography (HPLC). Gel filtration revealed two major immunoreactive peaks corresponding to the previously described 5-kDa and 8-kDa molecular forms, which appeared similar in both species. Isocratic reverse-phase HPLC revealed that the major immunoreactive GIP peak (5-kDa) in the human tissue eluted earlier than the corresponding porcine molecular form, indicating the latter to be less hydrophobic. These findings suggest significant species differences between human and porcine GIP.

【 授权许可】

Unknown