Complete sequence of the gene encoding the largest subunit of RNA polymerase I of Trypanosoma brucei

FEBS Letters

Hammer, Andrea¹  Jess, Waldemar¹  Cornelissen, Albert W.C.A.¹ 

[1] Max-Planck-Institut für Biologie, Molecular Parasitology Unit, Spemannstrasse, 34, 7400, Tübingen, FRG

关键词: RNA polymerase I;    Largest subunit;    Nucleotide sequence;    Sequence homology;    (Trypanosoma brucei);    VSG;    variant surface glycoprotein;    ESAG;    expression site-associated gene;    Pol I;    Pol II and Pol III indicate the largest subunit of the respective RNA polymerase;   

DOI  :  10.1016/0014-5793(89)80029-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have set out to clone the trypanosomal gene encoding the largest subunit of RNA polymerase I. We screened a genomic library with a synthetic oligonucleotide probe encoding an eleven amino acid sequence motif, YNADFDGDEMN, which has been found in all eukaryotic RNA polymerase largest subunit genes analyzed so far. We isolated the Trp11 locus and determined the complete sequence of the gene encoded within this locus. The deduced amino acid sequence contains the highly conserved RNA polymerase domains as well as the previously identified RNA polymerase I-specific hydrophilic insertions. Therefore, the gene most closely resembles the largest subunit of RNA polymerase I.

【 授权许可】

Unknown