Interleukin 3 and phorbol ester stimulate tyrosine phosphorylation of overlapping substrate proteins

FEBS Letters

Farrar, William L.³  Martensen, Todd¹  Willette-Brown, Jami²  Ferris, Douglas K.² 

[1] National Institute on Alcohol Abuse and Alcoholism, Section on Immunology, 12501 Washington Ave, Rockville, MD 20852 USA;Program Resources Inc., Biological Carcinogenesis & Development Program, ESP, Frederick Cancer Research Facility, Frederick, MD 21701-1013, USA;Laboratory of Molecular Immunoregulation, Biological Response Modifiers, Program Division of Cancer Treatment, Frederick Cancer Research Facility, Frederick, MD 21701, USA

关键词: Interleukin 3;    Phorbol ester;    Tyrosine kinase;    Phosphorylation;   

DOI  :  10.1016/0014-5793(89)80273-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

FDC-P1 is a murine myeloid cell line that requires interleukin 3 (IL3) for survival and proliferation. While the biological effects of IL3 have been well described, the biochemical mechanisms of IL3 actions have only recently been examined. We have investigated whether IL3 or PMA stimulates phosphorylation of proteins on tyrosine as well as on serine/threonine residues as previously described [(1986) Blood 68, 906–913; (1987) Biochem. J. 244, 683–691]. Here we report that both IL3 and PMA stimulate the tyrosine phosphorylation of at least two proteins: pp70 and pp50 in FDC-P1 cells.

【 授权许可】

Unknown