Tyrosine kinase activity is involved in the protein kinase C induced expression ofinterleukin‐1β gene in monocytic cells

FEBS Letters

Hurme, Mikko¹  Palkama, Tessa¹  Matikainen, Sampsa¹ 

[1] Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland

关键词: Interleukin-1β;    THP-1 cell line;    Phorbol ester;    Tyrosine kinase;    Protein kinase C;    AP-1;    PMA;    phorbol 12-myristate-13-acetate;    PKC;    protein kinase C;    AP-1;    activating protein 1;    CAT;    chloramphenicol acetyl transferase;   

DOI  :  10.1016/0014-5793(93)80045-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of protein tyrosine kinases in the expression of interleukin-1β (IL-1β) gene in response to phorbol esters (PMA) in THP-1 cell line was investigated. Genistein, a specific tyrosine kinase inhibitor, inhibited PMA induced IL-1β protein and mRNA levels in THP-1 cells. Genistein did not have a significant effect on PMA induced activity in transient transfection assays using reporter gene constructs containing the PMA responsive sequence of the IL- 1β gene linked to IL- 1β promoter, or five repeats of PMA responsive sites (AP-1 sites) in front of a thymidine kinase promoter. This indicates that the tyrosine kinase activity required for the PMA induced IL-1β expression is coupled downstream from or functions independent of the PMA induced AP-1 activity.

【 授权许可】

Unknown