Two physiological substrate‐specific casein kinases are present in the bovine mammary gland

FEBS Letters

Brooks, Charles L.¹ 

[1] Department of Veterinary Pathobiology, The Ohio State University, Columbus, OH 43210, USA

关键词: Casein;    Protein kinase;    Calmodulin;    (Bovine);   

DOI  :  10.1016/0014-5793(89)80167-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two species of casein kinase from lactating bovine mammary gland have been identified; a Ca²⁺- and CM-independent casein kinase and a Ca²⁺- and CM-dependent casein kinase. The Ca²⁺- and CM-independent casein kinase phosphorylates previously dephosphorylated α_s1-, β- or κ-casein while the Ca²⁺- and CM-dependent casein kinase prefers previously dephosphorylated β- or κ-casein as substrates. Two activities are indicated by their substrate specificity, sensitivity to Ca²⁺ and CM, pH maxima, and differential solubilization by anionic detergents. The presence of a regulated casein kinase in the lactating mammary gland suggests that casein phosphorylation may be a regulator of micelle formation or secretion.

【 授权许可】

Unknown