Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr‐specific protein kinase

FEBS Letters

Stocchetto, Silvia¹  Carignani, Giovanna¹  Pinna, Lorenzo A¹  Marin, Oriano¹ 

[1] Dipartimento di Chimica Biologica, Università di Padova, Centro per lo Studio delle Biomembrane del C.N.R., Padua, Italy

关键词: Protein kinase;    piD261;    Casein;    Osteopontin;    (Saccharomyces cerevisiae);    PKA;    cyclic AMP-dependent protein kinase;    PCR;    polymerase chain reaction;    PAGE;    polyacrylamide gel electrophoresis;    FMOC;    9-fluorenylmethoxycarbonyl;    Cdk;    cell cycle-dependent protein kinase;   

DOI  :  10.1016/S0014-5793(97)00980-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Saccharomyces cerevisiae YGR262c gene, whose disruption causes severely defective growth, encodes a putative protein kinase shorter than any other protein kinase biochemically characterized to date and lacking some of the conserved features of these enzymes. Here we show that the product of the YGR262c gene, piD261, expressed in E. coli with a C-terminal (His)₆ tag, is a bona fide Ser/Thr protein kinase as judged from its capability to autophosphorylate and to phosphorylate casein and osteopontin in the presence of [γ-³²P]ATP. In contrast, no phosphorylation of histones, myelin basic protein, phosvitin, bovine serum albumin and poly(Glu/Tyr)4:1 could be detected. Mn²⁺ or, less effectively, Co²⁺ are required for piD261 catalytic activity, which is conversely undetectable in the presence of Mg²⁺, a behaviour unique among Ser/Thr protein kinases.

【 授权许可】

Unknown