Isolation and characterisation of GLP‐1 7–36 amide from rat intestine Elevated levels in diabetic rats

FEBS Letters

Williams, G.¹  Kanse, S.¹  Kreymann, B.¹  Bloom, S.R.¹  Yiangou, Y.¹  Ghatei, M.A.¹ 

[1] Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 0NN, England

关键词: Glucagon-like peptide-1;    Chromatography;    Mass spectrometry;    Diabetes mellitus;    Peptide termination factor;    (Rat);   

DOI  :  10.1016/0014-5793(88)81008-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH₂, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH₂-like immunoreactivity (GLP-1 7–36 NH₂ IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH₂ IR in the colon.

【 授权许可】

Unknown