期刊论文详细信息
FEBS Letters
Isolation and characterisation of GLP‐1 7–36 amide from rat intestine Elevated levels in diabetic rats
Williams, G.1  Kanse, S.1  Kreymann, B.1  Bloom, S.R.1  Yiangou, Y.1  Ghatei, M.A.1 
[1] Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 0NN, England
关键词: Glucagon-like peptide-1;    Chromatography;    Mass spectrometry;    Diabetes mellitus;    Peptide termination factor;    (Rat);   
DOI  :  10.1016/0014-5793(88)81008-1
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH2-like immunoreactivity (GLP-1 7–36 NH2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH2 IR in the colon.

【 授权许可】

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