Development of porous polymer monoliths for reverse-phase chromatography of proteins.

Shepodd, Timothy J. (Sandia National Laboratories, Albuquerque, NM) ; Stephens, Christopher P. (Sandia National Laboratories, Albuquerque, NM)

Sandia National Laboratories

关键词: Acrylates;    Chromatography;    Polymers;    59 Basic Biological Sciences;    Proteins;   

DOI  :  10.2172/918341
RP-ID  :  SAND2003-8517
RP-ID  :  AC04-94AL85000
RP-ID  :  918341

美国|英语

来源: UNT Digital Library

【 摘 要 】

The polymers developed in this project are intended for use as a stationary phase in reverse-phase chromatography of proteins, where the mobile phase is a solution of acetonitrile and a phosphate buffer, 6.6 pH. A full library of pore sizes have been developed ranging from 0.41{micro}m to 4.09 {micro}m; these pore sizes can be determined by the solvent ratio of tetrahydrofuran:methoxyethanol during polymerization. A column that can separate proteins in an isocratic mode would be a vast improvement from the common method of separating proteins through gradient chromatography using multiple solvents. In the stationary phase, the main monomers have hydrophobic tails, lauryl acrylate and steryl acrylate. Separations of small hydrophobic molecules and peptides (trial molecules) have efficiencies of 24,000-33,000 theoretical plates m{sup -1}. The combination of a highly non-polar stationary phase and a mobile phase where the polarity can be controlled provide for excellent separation.