Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream ( Sparus aurata )

PeerJ

article

Ruben Gil-Solsona¹  Jaime Nácher-Mestre¹  Leticia Lacalle-Bergeron¹  Juan Vicente Sancho¹  Josep Alvar Calduch-Giner²  Félix Hernández¹  Jaume Pérez-Sánchez² 

[1] Research Institute for Pesticides and Water ,(IUPA), University Jaume I;Institute of Aquaculture Torre de la Sal

关键词: Aquaculture;    Nutrition;    Chromatography;    Mass spectrometry;    Gilthead sea bream;    Serum metabolomics;   

DOI  :  10.7717/peerj.2920

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

A metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. More than 15,000 different m/z ions were detected and Partial Least Squares–Discriminant analysis allowed a clear differentiation between the two experimental groups (fed and 10-day fasted fish) with more than 90% of total variance explained by the two first components. The most significant metabolites (up to 45) were elucidated on the basis of their tandem mass spectra with a broad representation of amino acids, oligopeptides, urea cycle metabolites, L-carnitine-related metabolites, glutathione-related metabolites, fatty acids, lysophosphatidic acids, phosphatidylcholines as well as biotin- and noradrenaline-related metabolites. This untargeted approach highlighted important adaptive responses in energy and oxidative metabolism, contributing to identify robust and nutritionally-regulated biomarkers of health and metabolic condition that will serve to assess the welfare status of farmed fish.

【 授权许可】

CC BY