PeerJ | |
Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream ( Sparus aurata ) | |
article | |
Ruben Gil-Solsona1  Jaime Nácher-Mestre1  Leticia Lacalle-Bergeron1  Juan Vicente Sancho1  Josep Alvar Calduch-Giner2  Félix Hernández1  Jaume Pérez-Sánchez2  | |
[1] Research Institute for Pesticides and Water ,(IUPA), University Jaume I;Institute of Aquaculture Torre de la Sal | |
关键词: Aquaculture; Nutrition; Chromatography; Mass spectrometry; Gilthead sea bream; Serum metabolomics; | |
DOI : 10.7717/peerj.2920 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
【 摘 要 】
A metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. More than 15,000 different m/z ions were detected and Partial Least Squares–Discriminant analysis allowed a clear differentiation between the two experimental groups (fed and 10-day fasted fish) with more than 90% of total variance explained by the two first components. The most significant metabolites (up to 45) were elucidated on the basis of their tandem mass spectra with a broad representation of amino acids, oligopeptides, urea cycle metabolites, L-carnitine-related metabolites, glutathione-related metabolites, fatty acids, lysophosphatidic acids, phosphatidylcholines as well as biotin- and noradrenaline-related metabolites. This untargeted approach highlighted important adaptive responses in energy and oxidative metabolism, contributing to identify robust and nutritionally-regulated biomarkers of health and metabolic condition that will serve to assess the welfare status of farmed fish.
【 授权许可】
CC BY
【 预 览 】
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RO202307100014375ZK.pdf | 2423KB | download |