FEBS Letters | |
Insulin‐induced decrease in 5′‐nucleotidase activity in skeletal muscle membranes | |
Cartee, Gregory D.1  Holloszy, John O.1  Douen, Andre G.2  Klip, Amira2  Ramlal, Toolsie2  Burdett, Elena2  Young, Douglas1  | |
[1] Department of Medicine, Washington University School of Medicine, St. Louis, MO 63122, USA;Department of Cell Biology, The Hospital for Sick Children, Toronto, M5G 1X8 Canada | |
关键词: 5′-Nucleotidase; Insulin action; Anchored protein; (Skeletal muscle); | |
DOI : 10.1016/0014-5793(88)80524-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Insulin releases inositol phosphoglycans from myocytes in culture [(1986) Science 233, 967–972], which display insulinomimetic activity. Because 5′-nucleotidase is anchored to the membrane through inositol-containing phospholipid glycans, we investigated whether insulin could release the enzyme from the membrane. Membranes prepared from hindquarter muscles of rats perfused with insulin showed a 23% decrease in 5′-nucleotidase activity. Isolated membranes from muscle exposed to insulin in vitro also showed a small but reproducible decrease (9%) in 5′-nucleotidase activity relative to unexposed controls. Phospholipase C from Staphylococcus aureus released 60% of the membrane-bound 5′-nucleotidase. We propose that insulin may activate an endogenous phospholipase C that cleaves phospholipid-glycan-anchored proteins.
【 授权许可】
Unknown
【 预 览 】
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