Insulin‐induced decrease in 5′‐nucleotidase activity in skeletal muscle membranes

FEBS Letters

Cartee, Gregory D.¹  Holloszy, John O.¹  Douen, Andre G.²  Klip, Amira²  Ramlal, Toolsie²  Burdett, Elena²  Young, Douglas¹ 

[1] Department of Medicine, Washington University School of Medicine, St. Louis, MO 63122, USA;Department of Cell Biology, The Hospital for Sick Children, Toronto, M5G 1X8 Canada

关键词: 5′-Nucleotidase;    Insulin action;    Anchored protein;    (Skeletal muscle);   

DOI  :  10.1016/0014-5793(88)80524-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin releases inositol phosphoglycans from myocytes in culture [(1986) Science 233, 967–972], which display insulinomimetic activity. Because 5′-nucleotidase is anchored to the membrane through inositol-containing phospholipid glycans, we investigated whether insulin could release the enzyme from the membrane. Membranes prepared from hindquarter muscles of rats perfused with insulin showed a 23% decrease in 5′-nucleotidase activity. Isolated membranes from muscle exposed to insulin in vitro also showed a small but reproducible decrease (9%) in 5′-nucleotidase activity relative to unexposed controls. Phospholipase C from Staphylococcus aureus released 60% of the membrane-bound 5′-nucleotidase. We propose that insulin may activate an endogenous phospholipase C that cleaves phospholipid-glycan-anchored proteins.

【 授权许可】

Unknown