Redistribution of membrane 5′‐nucleotidase in rabbit peritoneal polymorphonuclear leucocytes during phagocytosis

FEBS Letters

Crawford, N.²  Stewart, D.I.H.¹ 

[1] Department of Biochemistry, University of Alberta, Edmonton, Canada;Department of Biochemistry, Royal College of Surgeons of England, Lincoln's Inn Fields, London, WC2A 3PN, Canada

关键词: Phagocytosis;    5′-Nucleotidase;    Redistribution;    Surface membrane domain;   

DOI  :  10.1016/0014-5793(83)80523-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a quantitative phagocytic model involving oil droplet internalisation rabbit polymorphonuclear leucocytes display selective segregation of membrane constituents during the phagocytic event. A resting cell surface membrane and fractions representing vesicle membranes and uninvolved surface membranes from the active cells have been purified by density gradient sedimentation and free flow electrophoresis. The specific activity of 5′-nucleotidase, a major neutrophil surface membrane glycoprotein, was 3-fold higher in the univolved membrane of phagocytosing cells than in the resting cell membrane. The activity in the vesicle membranes was substantially depleted. In contrast Lens culinaris receptors showed no redistribution during phagocytosis: the two surface domains showing essentially the same enrichment with respect to homogenate as the resting cell surface membrane.

【 授权许可】

Unknown