5′‐Nucleotidase is activated upon cholesterol‐depletion of liver plasma membranes

FEBS Letters

Houslay, Miles D.¹  Whetton, Anthony D.¹ 

[1] Department of Biochemistry and Applied Molecular Biology, UMIST, PO Box 88, Manchester M6O 1QD, England

关键词: 5′-Nucleotidase;    Cholesterol;    Plasma membrane;    Lipid—protein interaction;   

DOI  :  10.1016/0014-5793(83)81118-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cholesterol content of rat liver plasma membranes was manipulated using either cholesterol-free or cholesterol-enriched liposomes. Removal of cholesterol from the membranes led to a marked increase in 5′-nucleotidase activity. However, increase in cholesterol content failed to exert any significant effect on 5′-nucleotidase activity. Arrhenius plots of the activity of the native enzyme exhibited a break at around 28°C with the activation energy of the reaction less above this temperature than below. In cholesterol-depleted membranes a single break at around 26°C was observed with activation energies greater above this temperature than below it. In cholesterol-enriched membranes Arrhenius plots were linear over the range examined. It is suggested that the lipid environment of the external half of the bilayer only influences 5′-nucleotidase activity in these membranes and that cholesterol exerts controlling effects on both the activity and conformation of the enzyme in native membranes.

【 授权许可】

Unknown