Insulin‐induced translocation of glucose transporters in rat hindlimb muscles

FEBS Letters

Young, Douglas A.¹  Holloszy, John O.¹  Klip, Amira²  Ramlal, Toolsie² 

[1] Department of Medicine, Washington University School of Medicine, St. Louis, MO 63122, USA;Division of Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada

关键词: Glucose transport;    Insulin action;    (Skeletal muscle);   

DOI  :  10.1016/0014-5793(87)80452-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions from insulinized and control muscles. Insulin treatment caused an approx. 2-fold increase in cytochalasin B-binding sites in a plasma membrane fraction and an approx. 70% decrease in cytochalasin B-binding sites in an intracellular membrane fraction. In order to detect this effect of insulin, it was necessary to develop a procedure for isolating a plasma membrane fraction and an intracellular membrane fraction that were not contaminated with sarcoplasmic reticulum. Our results show that, as in adipocytes, insulin stimulates translocation of glucose transporters from an intracellular membrane pool to the plasma membrane in hindlimb skeletal muscles.

【 授权许可】

Unknown