High level of expression in the prostate of a human glandular kallikrein mRNA related to prostate‐specific antigen

FEBS Letters

Paradis, Gilles¹  Tremblay, Roland R.¹  Dubé, Jean Y.¹  Chapdelaine, Pierre¹ 

[1] Hormonal Bioregulation Laboratory, Laval University Hospital Centre, 2705 Laurier Boulevard, Ste-Foy, Quebec G1V 4G2, Canada

关键词: Prostate cancer;    Proteolytic enzyme;    Trypsin-like enzyme;    Chymotrypsin-like enzyme;    PSA;    prostate-specific antigen;    hGK-1;    human glandular kallikrein-1;    RAV2;    Rous associated virus 2;    1 × Mops;    20 mM morpholinopropanesulfonic acid;    1 × SSC;    0.15 M NaCl;    0.015 M sodium citrate;    pH 7.0;   

DOI  :  10.1016/0014-5793(88)80315-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a synthetic oligonucleotide primer complementary to human prostate-specific antigen mRNA, we found that an additional sequence possibly similar to human glandular kallikrein-1 could be read by a primer-extension sequencing technique. We were able to confirm the identity of that additional sequence with another oligonucleotide primer complementary to a specific region of the human glandular kallikrein-1 mRNA sequence. Northern blot analysis with 2 oligonucleotide probes respectively specific for prostate-specific antigen and human glandular kallikrein-1 mRNAs showed that the length of both mRNAs was similar at 1.5 kb. The level of human glandular kallikrein-1 mRNA relative to that of prostate-specific antigen could be estimated as approx. 10–20%. This study constitutes the first evidence that the human glandular kallikrein-1 gene is expressed at a high level in a human tissue.

【 授权许可】

Unknown