Preclinical increase in activity of muscle microsomal trypsin‐like protease in murine muscular dystrophy, C57BL/10‐mdx

FEBS Letters

Sawada, Hitoshi¹  Kusumoto, Shigeo¹  Tsuji, Shigekatsu¹  Doi, Yoshio¹  Matsushita, Hiroshi¹ 

[1] Department of Physiology, Wakayama Medical College, Kyuban-cho 9, Wakayama 640, Japan

关键词: Muscular dystrophy;    Protease;    Trypsin-like enzyme;    Enzyme activity;    (mdx mouse);   

DOI  :  10.1016/0014-5793(86)80478-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preclinical alterations of protease activities in skeletal muscles from 10-day-old dystrophic mouse, C57BL/10-mdx, were examined by using 10 fluorogenic peptide substrates. Among the activities tested, only Boc-Val-Pro-Arg-MCA-hydrolyzing enzyme of the muscle microsomes showed an about 6-fold higher level of activity in mdx mouse. The increase in activity was not observed in tissues other than skeletal muscle. The enzyme had a pH optimum between 8.5 and 11.0, and was inhibited with DFP and variety of trypsin inhibitors. The enzymatic activity transiently increased at 1-2 weeks of age, the preclinical or very early stage of the disease. These results imply that the increased level of a trypsin-like protease possibly present in muscle microsomes may be closely related to the manifestation of muscular dystrophy.

【 授权许可】

Unknown