The chymotrypsin‐like activity of human prostate‐specific antigen, γ‐seminoprotein

FEBS Letters

Nakamura, Takanori²  Hara, Mitsuwo¹  Iwanaga, Sadaaki²  Akiyama, Kazuko¹ 

[1] Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830, Japan;Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka 812, Japan

关键词: Prostate-specific antigen;    Seminal plasma protease;    Chymotrypsin-like enzyme;    γ-Seminoprotein;    γ-Sm;    γ-seminoprotein;    RSA-lysozyme;    reduced and S-3-(trimethylated amino)propylated lysozyme;    pNA;    p-nitroanilide;   

DOI  :  10.1016/0014-5793(87)81151-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

γ-Seminoprotein (γ-Sm) is a human prostate-specific antigen and a serine protease judging from the complete amino acid sequence which shows extensive homology with the kallikrein family. The enzymatic activity of γ-Sm was defined as a chymotypsin-like activity using reduced and S-3-(trimethylated amino)propylated lysozyme and insulin-oxidized A and B chains as substrates. The -LeuSer- peptide bond of lysozyme was rapidly hydrolyzed by γ-Sm. γ-Sm also hydrolyzed the -PheGlu- of lysozyme and the -LeuCys(SO₃H) -of insulin B chain. Insulin A chain and arginyl- or lysyl-linkage of these proteins were not hydrolyzed by γ-Sm at all.

【 授权许可】

Unknown