Intracellular distribution of DNA methyltransferase during the cell cycle

FEBS Letters

Müller-Hermelink, H.K.²  Papadopoulos, T.²  Pfeifer, G.P.¹  Drahovsky, D.¹  Vogel, M.C.¹ 

[1] Center of Biological Chemistry, University of Frankfurt, D-6000 Frankfurt a.M. FRG;Institute of Pathology, University of Würzburg, D-8700 Würzburg, FRG

关键词: DNA methyltransferase;    DNA methylation;    Immunocytochemistry;    Cell cycle;   

DOI  :  10.1016/0014-5793(88)80275-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nuclei with weak cytoplasmic staining. The DNA methyltransferase antigen was absent in early G₁ phase, appeared in late G₁ prior to the onset of DNA synthesis and persisted throughout S and G₂ phases of the cell cycle. Mitotic cells showed a particularly strong staining intensity. These results show that DNA methyltransferase levels fluctuate during the cell cycle. This has possible implications on the stability of the DNA methylation pattern.

【 授权许可】

Unknown