DNA methyltransferase activity in the early stages of a sea urchin embryo Evidence of differential control

FEBS Letters

Aniello, Francesco¹  Tosi, Luisa¹  Branno, Margherita¹  Geraci, Giuseppe² 

[1] Laboratory of Biochemistry and Molecular Biology, Zoological Station ‘A. Dohrn’, Villa Comunale, 80121 Naples, Italy;Department of Genetics, General and Molecular Biology, University of Naples ‘Federico II’, Naples, Italy

关键词: DNA methyltransferase;    Enzyme control;    Development (Sphaerechinus granularis);    Proteolysis;    DNA methylation;    S-Adenosylmethionine;    AdoMet;    S-Adenosylmethionine DNA(cytosine-5) methyltransferase (E.C. 2.1.1.37);    DNA-MTase;   

DOI  :  10.1016/0014-5793(95)00160-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The specific activity of DNA methyltransferase increases in the nuclei of Sphaerechinus granularis sea urchin embryos at increasing stages of development. The activity reaches maximal value at about 20 h of growth, when embryos are at the mesenchyme blastula stage, then abruptly decreases and is essentially zero at about 35 h of development, when embryos are at the early gastrula stage. Both the increase and the drop of the activity are faster than embryonic cell duplication indicating that the enzyme is under strict control during development and that, in the more advanced embryo, a mechanism is activated to specifically block its activity.

【 授权许可】

Unknown