Preferential binding of DNA methyltransferase and increased de novo methylation of deoxyinosine containing DNA

FEBS Letters

Drahovsky, Dusan¹  Pfeifer, Gerd P.¹ 

[1] Zentrum der Biologischen Chemie der Universität Frankfurt, D-6000 Frankfurt am Main, FRG

关键词: DNA methyltransferase;    Methylation;    Poly(dI-dC)·poly(dI-dC);   

DOI  :  10.1016/0014-5793(86)80015-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mammalian DNA-cytosine 5-methyltransferases methylate cytosines in deoxyinosine containing DNA polymers more rapidly than in other synthetic or naturally occurring DNAs. The initial methylation rate of poly(dI-dC)·poly(dI-dC) is about 10-times higher than that of poly-(dG-dC)·poly(dG-dC) or of the native Micrococcus luteus DNA. In competitive binding experiments, DNA methyltransferase has about 10-fold higher affinity for the dI-containing alternating DNA polymer than for poly(dG-dC) · poly(dG-dC). The observed high methyl accepting capacity of poly(dI-dC) · poly(dI-dC) may be a useful methodological advance to determine de novo DNA methyltransferase activity in extracts of mammalian cells.

【 授权许可】

Unknown