Phorbol ester modulates serotonin receptor‐mediated increases in inositol phosphate production and calcium mobilization in cultured rat vascular smooth muscle cells

FEBS Letters

Hirata, Yukio²  Takata, Shoichiro²  Takagi, Yasuyuki²  Yoshimi, Hiroki²  Fukuda, Yuka²  Fujita, Takuo¹ 

[1] Third Division, Department of Medicine, Kobe University School of Medicine, Kobe 650, Japan;Hyptertension-Endocrine Division, National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

关键词: Serotonin;    Inositol phosphate;    Protein kinase C;    Intracellular Ca2+ concentration;    Phorbol ester;    (Vascular smooth muscle cell);   

DOI  :  10.1016/0014-5793(88)81340-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on serotonin-induced inositol phosphate (IP) accumulation and intracellular free Ca²⁺ concentrations ([Ca²⁺]_i) was investigated in cultured rat vascular smooth muscle cells. Pretreatment with TPA had no effect on basal levels of both IP production and [Ca²⁺]_i, whereas it significantly attenuated serotonin-induced increases in both IP production and [Ca²⁺]_i. These data suggest that protein kinase C is involved in the negative feedback control of serotonin-induced rises in both IP production and [Ca²⁺]_i.

【 授权许可】

Unknown