Tetanus toxin‐induced protein kinase C activation and elevated serotonin levels in the perinatal rat brain

FEBS Letters

Aguilera, Jose²  Lopez, Lluis A.¹  Yavin, Ephraim² 

[1] Department of Biochemistry and Molecular Biology, Universidad Autonoma de Barcelona, Cerdanyola, Spain;Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel

关键词: Tetanus toxin;    Serotonin;    Protein kinase C;    Ganglioside;    Brain development;    H-7;    1-(5-isoquinolinylsulfonyl)-2-methylpiperazine;    GM 1 galactosyl-N-acetylgalactosaminyl-(N acetylneuraminyl)-galactosylglucosylceramide;    GDlb galactosyl-Macetylgalactosaminyl-(N-acetylneuraminyl-N-acetymeuraminyl)galactosylglucosylceramide;    BBG;    bovine brain ganglioside mixture;    TPA;    12-O-tetra-decanoyl-phorbol-13-acetate;    PKC Ca2+-phosphatidylserinedependent protein kinase C;    TeTox;    tetanus toxin;   

DOI  :  10.1016/0014-5793(90)80705-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A single intracerebral injection of tetanus toxin (TeTox) is able to produce a time-dependent translocation of Ca²⁺-phosphatidylserine-dependent protein kinase C (PKC) in close-to-tenn rat brain. TeTox-triggered translocation of PKC is dose- and time-dependent, can be prevented by tetanus antitoxin, and does not occur upon administration of toxin fragments B and C. TeTox-triggered PKC translocation is accompanied by a timedependent increase in brain serotonin (5-HT). Increase of brain 5-HT is independent of monoamine oxidase inhibition by pargyline. Phorbol ester and TeTox cause a significant increase in serotonin while H-7, a kinase inhibitor, does not affect serotonin levels but abolishes the effect of TeTox. Gangliosides prevent TeTox-triggered 5-HT increase. The data are consistent with the possibility that TeTox acts effectively on the serotonergic innervation, presumably in conjunction with PKC to cause accumulation of serotonin.

【 授权许可】

Unknown