Phorbol esters inhibit inositol phosphate and diacylglycerol formation in proliferating HL60 cells Relationship to differentiation

FEBS Letters

Geny, Blandine¹  Basset, Michele¹  LePeuch, Christian¹  Cockcroft, Shamshad¹  Cost, Helene¹ 

[1]INSERM, Unite´204, Centre Hayem, Hopital Saint Louis, 75475 Paris Cedex 10, France

关键词: Protein kinase C;    Inositol phosphate;    Diacylglycerol;    Phorbol 12-myristate 13-acetate;    Differentiation;    (HL60 cell);    OAG;    1-oleoyl-2-acetyl-sn-glycerol;    PMA;    phorbol 12-myristate 13-acetate;    PDBu;    phorbol 12;    13-dibutyrate;    PRA;    phorbol 12-retinoate 13-acetate;    4αPDD;    4α-phorbol 12;    13-didecanoate;    IPs;    total inositol phosphates;    IP3;    inositol trisphosphate;    DG;    diacylglycerol;   

DOI  :  10.1016/0014-5793(88)80434-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phorbol 12-myristate 13-acetate (PMA) induces the differentiation of the human promyelocytic cell line, HL60, towards adherent macrophage-like cells within 2 days. We have examined the early effects of PMA on inositol phosphates and on diacylglycerol production, two second messengers derived from inositol lipids. In proliferating HL60 cells, PMA induced a time- and concentration-dependent decrease in inositol phosphate levels. Maximal effects were seen after 1 h at 10 nM PMA. PMA also induced the translocation of protein kinase C from the cytosol to the membrane. Comparison between the differentiating effects of several phorbol esters and of 1-oleoyl-2-acetylglycerol with their ability to inhibit inositol phosphate formation suggests that the two effects are correlated.

【 授权许可】

Unknown