Breakdown and synthesis of polyphosphoinositides in fMetLeuPhe‐stimulated neutrophils

FEBS Letters

Barrowman, M.M.¹  Gomperts, B.D.¹  Cockcroft, S.¹ 

[1]Department of Experimental Pathology, University College London, University Street, London WC1E 6JJ, England

关键词: Neutrophil;    Receptor;    Polyphosphoinositide;    Inositol phosphate;    Diacylglycerol;    Phospholipase C Phospholipase D;    PI;    phosphatidylinositol;    PPI;    polyphosphoinositide (includes PI-P;    phosphatidylinositol 4-phosphate and PI-P2;    phosphatidylinositol 4;    5-bisphosphate);    DG;    diacylglycerol;    PA;    phosphatidate;    PMA;    phorbol myristate acetate;    fMet;    formylmethionyl;    pde;    phosphodiesterase;    IPs;    inositol phosphates (includes IP2 inositol bisphosphate and IP3;    inositol trisphosphate);   

DOI  :  10.1016/0014-5793(85)80271-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interconversions of the inositol-containing lipids (PI, PI-P and PI-P₂) and their products (DG, inositol phosphates and PA) in human and rabbit neutrophils stimulated with fMetLeuPhe and PMA have been examined. PMA causes only the phosphorylation of PI to PI-P whereas fMetLeuPhe causes phosphorylation of both PI and PI-P yielding PI-P₂ and the hydrolysis of all three lipids. While the predominant reaction is breakdown of PI to PA catalysed by phospholipase D, approx. 2% of PI is converted to polyphosphoinositides and then broken down by the phospholipase C route yielding inositol phosphates and DG. The latter reaction occurs without detectable lag and is a function of receptor occupancy. The amount of inositol trisphosphate thus formed would be sufficient to release Ca²⁺ from intracellular stores.

【 授权许可】

Unknown