Recognition of envelope and tat protein synthetic peptide analogs by HIV positive sera or plasma

FEBS Letters

Cumming, Simon¹  Kemp, Bruce E.¹  Gust, Ian D.¹  Doherty, Richard R.¹  McPhee, Dale A.¹  Stapleton, David² 

[1] NHMRC Special Unit for AIDS Virology, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Fairfield Australia;Department of Medicine, University of Melbourne, Repatriation General Hospital, Heidelberg, Victoria, Australia

关键词: HIV;    TatIII;    Predicted antigenicity;    Antibody response;    Synthetic peptide;   

DOI  :  10.1016/0014-5793(88)80468-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A series of synthetic peptides corresponding to segments of HIV encoded proteins were selected using criteria described by Welling et al. [(1985) FEBS Lett. 188, 215]. Synthetic peptide analogs to gpl20 (2–13), (55–65), gp41 (582–596) (659–670) and tat_III (71–83) were recognized by 41–67% of sera or plasma from individuals known to be infected with HIV on the basis of virus isolation or Western blot screening. The peptide which reacted with most sera or plasma was gp41 (582–596), a conserved region in the transmembrane glycoprotein. An extended peptide analog, gp41 (579–599), tested against the same samples showed almost 100% reactivity, confirming independent studies identifying a highly immunodominant region of gp41. There was an unexpected high prevalence of antibodies (52%) to the tat_III peptide.

【 授权许可】

Unknown