The ATP‐driven primary Na+ pump in subcellular vesicles of Vibrio alginolyticus

FEBS Letters

Dibrov, P.A.¹  Sokolov, M.V.¹  Skulachev, V.P.¹  Verkhovskaya, M.L.¹ 

[1] Department of Bioenergetics, A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Na+-ATPase;    Na+ bioenergetics;    Bacterial vesicle;    (Vibrio alginolyticus);    ΔgmH;    ΔgmNa;    electrochemical H+;    Na+;    potential difference respectively;    CCCP;    m-chlorocarbonylcyanide phenylhydrazone;    DCCD;    N;    N′-dicyclohexylcarbodiimide;   

DOI  :  10.1016/0014-5793(88)80459-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subcellular vesicles of Vibrio alginolyticus hydrolyse ATP and accumulate Na⁺ in an ATP-dependent fashion. The Na⁺ uptake is (i) strongly stimulated by Δψ-discharging agents, i.e., the protonophorous uncoupler CCCP or valinomycin +K⁺ and (ii) arrested by DCCD at a concentration strongly inhibiting ATP hydrolysis. Lower concentrations of DCCD stimulate the Na⁺ accumulation supported by ATP hydrolysis as well as by NADH oxidation. It is concluded that there is an electrogenic DCCD-sensitive Na⁺-ATPase in the cytoplasmic membrane of V. alginolyticus.

【 授权许可】

Unknown