The ATP‐dependent generation of membrane potential by sub‐bacterial vesicles from the marine bacterium, Vibrio alginolyticus

FEBS Letters

Kostyrko, V.A.¹  Smirnova, Irina A.¹  Vulfson, E.N.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Membrane potential;    ATPase;    (Vibrio alginolyticus);    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    HQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    PCB−;    phenyldicarbaundecaborane anion;    Δµ̄;    Δµ̄Na+;    electrochemical gradients of H+ and Na+;    respectively;    Δψ;    transmembrane electric potential difference;   

DOI  :  10.1016/0014-5793(87)80084-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of ATP leads to the accumulation of the permeant anion PCB⁻ by sub-bacterial vesicles from Vibrio alginolyticus. This accumulation is caused by Δψ generation by ATPase, the effect being inhibited by CCCP, gramicidin D and DCCD. Δψ values may be increased by incubation of sub-bacterial vesicles at room temperature and with the protein fraction isolated according to Beechey et al. [(1975) Biochem. J. 148, 533–537] from another portion of the sub-bacterial vesicles. Δψ generation is observable only in the presence of Mg²⁺ at high concentrations (optimum ≈ 30 mM). Proceeding from experimental data we assume that Mg²⁺ reduces passive H⁺ conductivity of the vesicle membranes. Thus, a Δψ-generating ATPase has been shown for the first time in V. alginolyticus membranes.

【 授权许可】

Unknown