Isolation and identification of hydroxyproline analogues of bradykinin in human urine

FEBS Letters

Maeda, Hirosi¹  Kato, Hisao²  Matsumura, Yasuhiro¹ 

[1] Department of Microbiology, Kumamoto University Medical School, Kumamoto 860, Japan;National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

关键词: Kinin;    Hydroxyproline;    Bradykinin;    Kallikrein;    Lysyl-bradykinin;    (Human urine);    BK;    bradykinin;    Hyp;    hydroxyproline;   

DOI  :  10.1016/0014-5793(88)80427-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hydroxyproline (Hyp) analogues of bradykinin and lysyl-bradykinin, in which the third residue of bradykinin, proline, is replaced by hydroxyproline, were isolated from human urine. Their amino acid sequences were confirmed by both amino acid and sequence analyses, and also by comparison of their chromatographic behavior with that of synthetic peptides. The possibility that Lys-Ala³-bradykinin, isolated by Mindroiu et al. [(1986) J. Biol. Chem. 261, 7407-7411] from human urine, was actually Lys-Hyp³-bradykinin is discussed.

【 授权许可】

Unknown