Identification of [hydroxyproline3]‐lysyl‐bradykinin released from human kininogens by human urinary kallikrein

FEBS Letters

Binder, Bernd R.²  Reissert, Guenther²  Jerabek, Ingrid²  Maier, Manfred²  Lottspeich, F.¹ 

[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG;Laboratory for Clinical and Experimental Physiology, Department of Medical Physiology, University of Vienna, Vienna, Austria

关键词: Kininogen;    Kallikrein;    Kinin;    [Hyp3]-lys-bradykinin;   

DOI  :  10.1016/0014-5793(88)80778-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The types of kinins released from purified native, single chain human high and low molecular mass kininogens (HMMKs and LMMKS, respectively) by purified human urinary kallikrein were separated by reverse-phase HPLC and quantitated by the rat uterus bioassay. [Hyp³]-ysyl-bradykinin, a recently discovered kinin, represented up to 58% of the biological activity released from 4 individual HMMK preparations purified from 4 different healthy volunteers. In contrast, the majority of the biological activity released from LMMKs purified from pooled plasma was identified as Lys-bradykinin and [Hyp³]-lysyl-bradykinin represented only 6.4 ± 3.8%. These findings indicate posttranslational hydroxylation of human kininogens and suggest a preference of HMMKs for this modification.

【 授权许可】

Unknown