Hydroxyprolyl3‐bradykinin in high molecular mass kininogen presence in human and monkey kininogens, but not in kininogens from bovine, rat, rabbit, guinea pig and mouse plasmas

FEBS Letters

Enjyoji, Kei-ichi¹  Kato, Hisao¹ 

[1] National Cardiovascular Center Research Institute, Fujishirodai 5, Suita, Osaka 565, Japan

关键词: Kinin;    Hydroxyproline;    Kininogen;    (Human;    Monkey);    Hyp;    hydroxyproline;    HPLC;    high-performance liquid chromatography;    NBD-Cl;    7-chloro-4-nitrobenzo-2-oxa-1;    3-diazole chloride;    HMM;    high molecular mass;    BK;    bradykinin;   

DOI  :  10.1016/0014-5793(88)80212-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The contents of hydroxyprolyl³-bradykinin in high molecular mass (HMM) kininogens from human and animal plasmas were examined by reversed-phase HPLC following their proteolytic scission by bovine plasma kallikrein. The relative contents of hydroxyprolyl³-bradykinin in kinins from HMM kininogens from pooled plasmas of human and monkey origin were 33 and 73%, respectively. On the other hand, hydroxyprolyl³-bradykinin could not be detected in HMM kininogen preparations from bovine, rat, guinea pig, rabbit and mouse plasmas. Hydroxyproline in hydroxyprolyl³-bradykinin was assigned as trans-4-hydroxyproline by comparison of the retention times on reversed-phase HPLC with isomers of hydroxyproline after derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole chloride.

【 授权许可】

Unknown