Purification of a vasoactive peptide related to lysyl‐bradykinin from trout plasma

FEBS Letters

Conlon, J.Michael¹  Olson, Kenneth R.² 

[1] Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA;Indiana University School of Medicine, South Bend Center, University of Notre Dame, Notre Dame, IN 46556, USA

关键词: Bradykinin;    Kallidin;    Kininogen;    Kallikrein;    Blood pressure;    Trout;   

DOI  :  10.1016/0014-5793(93)81684-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe⁶→Trp and Phe⁹→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein—kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

【 授权许可】

Unknown