Phosphorylation of the 90 kDa heat shock protein in heat shocked HeLa cell lysates

FEBS Letters

Legagneux, Vincent²  Dubois, Marie-Françoise¹  Bensaude, Olivier²  Morange, Michel² 

[1] INSERM U.43 Hôpital St Vincent de Paul, 74, av. Denfert-Rochereau, 75014 Paris, France;Groupe de Biologie Moléculaire du Stress, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15 France

关键词: Heat shock;    Heat shock protein;    Protein phosphorylation;    Eukaryotic initiation factor;    Protein kinase;    (HeLa cell);   

DOI  :  10.1016/0014-5793(88)80862-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 90 kDa heat shock protein (hsp 90) is a major phosphorylated protein under normal growth conditions. However, it does not incorporate detectable levels of phosphate by incubation of control HeLa cell lysates with [γ-³²P]ATP in vitro. In this paper we show that strong phosphorylation of hsp 90 occurs in lysates prepared from heat shocked HeLa cells. Possible involvement of the eukaryotic initiation factor 2 kinase of the heme-controlled repressor of translation is discussed.

【 授权许可】

Unknown