Hsp70 as an indicator of stress in the cells after contact with nanoparticles

4th International Conference on Safe Production and Use of Nanomaterials

Hardilová, Šárka^1 ; Havrdová, Markéta^2 ; Panáek, Ale^1 ; Kvítek, Libor^1 ; Zboil, Radek^1

Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacky University, 17. listopadu 12, Olomouc

771 46, Czech Republic^1

Regional Centre of Advanced Technologies and Materials, Department of Experimental Physics, Faculty of Science, Palacky University, 17. listopadu 12, Olomouc

771 46, Czech Republic^2

关键词: Chaperone activity;    Denatured proteins;    Denaturing conditions;    Heat shock protein;    Low concentrations;    Low temperatures;    Mouse-fibroblasts;    Physiological condition;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/617/1/012023/pdf
DOI  :  10.1088/1742-6596/617/1/012023

来源: IOP

【 摘 要 】

In recent years, production of nanoparticles is increased and thus grows our contact with them too. Question of safety is closely related to the issue of use nanoparticles. There are a number of tests that monitor the viability, ROS production, the effect on the DNA and cell cycle, however, rarely encountered studies on stress in the cells after contact with nanoparticles. Heat shock proteins (HSP) are among the substances that can be used for monitoring stress in cells. HSP are structures with a chaperone activity. They are evolutionarily very old, conservative and they are found with a high degree of homology in prokaryotes and eukaryotes including humans. They exist at low concentrations under physiological conditions, while in the denaturing conditions e.g. high or low temperature, radiation, exposure to chemicals, heavy metals, or nanoparticles their expression is changed. HSPs are involved in maintaining homeostasis in the cell that the denatured protein conformations allow recovery to the original stage. One of the most common proteins from HSP family is Hsp70 - protein with a molecular weight of 70 kDa. The level of Hsp70 in a cell after exposure to the stress changes depending on the stress level to which the cell is exposed to and a time period during which lasted stressful conditions. Our research monitors stress levels of cells manifesting by Hsp70 production after contact with silver nanoparticles. Nanoparticles show different toxicity towards different types of target cells, which is reflected in the values of IC50- concentration that kills 50% tested cells. Concentration of test substance toxic to one cell type may be innocuous to cells of another type. IC50obtained from the MTT assay provides a suitable default data and if multiples of IC50values are used, we can compare and generalize. Studies can be used to compare stress levels in cells that show different sensitivity to the tested nanoparticles compared with cells under optimal growth conditions. The study was done on two types of mouse fibroblasts NIH-3T3 and L929. While NIH-3T3 cells exhibit stress response proportional to the concentration of silver nanoparticles, for L929 cells this was not observed.