FEBS Letters | |
Pertussis toxin reverses the inhibition of insulin secretion caused by [Arg8]vasopressin in rat pancreatic islets | |
Taylor, Keith W.1  Khalaf, Luma J.1  | |
[1] Biochemistry Department, The London Hospital Medical College, Turner Street, London E1 2AD, England | |
关键词: Vasopressin; cyclic AMP; Pertussis toxin; Islet of Langerhans; | |
DOI : 10.1016/0014-5793(88)80720-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
When rat pancreatic islets were incubated with 10−8 M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pertussis toxin (100 ng/ml) reversed the inhibition of insulin release due to vasopressin, indicating the possible involvement of a guanyl-nucleotide regulatory protein in the inhibitory effect. Nevertheless, 10−8 M vasopressin increased islet concentrations of cyclic AMP even under conditions where insulin release was decreased.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020290457ZK.pdf | 269KB | download |