Pertussis toxin reverses the inhibition of insulin secretion caused by [Arg8]vasopressin in rat pancreatic islets

FEBS Letters

Taylor, Keith W.¹  Khalaf, Luma J.¹ 

[1] Biochemistry Department, The London Hospital Medical College, Turner Street, London E1 2AD, England

关键词: Vasopressin;    cyclic AMP;    Pertussis toxin;    Islet of Langerhans;   

DOI  :  10.1016/0014-5793(88)80720-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When rat pancreatic islets were incubated with 10⁻⁸ M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pertussis toxin (100 ng/ml) reversed the inhibition of insulin release due to vasopressin, indicating the possible involvement of a guanyl-nucleotide regulatory protein in the inhibitory effect. Nevertheless, 10⁻⁸ M vasopressin increased islet concentrations of cyclic AMP even under conditions where insulin release was decreased.

【 授权许可】

Unknown