Elevated levels of the guanine nucleotide binding protein, Go, are associated with differentiation of neuroblastoma × glioma hybrid cells

FEBS Letters

Mullaney, Ian¹  Milligan, Graeme¹ 

[1] Molecular Pharmacology Group, Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland

关键词: Guanine nucleotide-binding protein;    cyclic AMP;    Differentiation;    ADP-ribosylation;    Pertussis toxin;   

DOI  :  10.1016/0014-5793(89)81174-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Each of a range of pharmacological agents which function to increase intracellular levels of cAMP caused a morphological ‘differentiation’ of neuroblastoma × glioma hybrid, NG108-15, cells grown in tissue culture. Associated with this differentiation, increased incorporation of [³²P]ADP-ribose catalysed by pertussis toxin was noted into a band of some 39–40 kDa in membranes derived from these cells. Immunoblotting using two antipeptide antisera which identify different regions of G_oα demonstrated marked increases in the levels of this polypeptide in membranes of the differentiated cells. However, levels of the β-subunit did not increase appreciably with differentiation.

【 授权许可】

Unknown