Functional expression of cloned cDNA encoding the α‐subunit of adenylate cyclase‐stimulating G‐protein

FEBS Letters

Mishina, Masayoshi¹  Nukada, Toshihide¹  Numa, Shosaku¹ 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan

关键词: Adenylate cyclase-stimulating G-protein;    cDNA;    β-Adrenergic agonist;    cyclic AMP;    Cholera toxin dependence;    ADP-ribosylation;    (cyc− S49 lymphoma cell);    G-protein;    guanine nucleotide-binding regulatory protein;    Gs;    adenylate cyclase-stimulating G-protein;    Gi;    adenylate cyclase-inhibiting G-protein;    SV40;    simian virus 40;    DMEM;    Dulbecco's modified Eagle's medium;    IBMX;    3-isobutyl-1-methylxanthine;   

DOI  :  10.1016/0014-5793(87)81263-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cloned cDNA encoding the α-subunit of the adenylate cyclase-stimulating G-protein (G_s), carried by a simian virus 40 vector, has been introduced into the cyc⁻ variant of S49 lymphoma cells by electroporation. In contrast to untransfected cys⁻ cells, clones transformed with the cDNA exhibit an increase in intracellular cyclic AMP concentration in response to a β-adrenergic agonist.

【 授权许可】

Unknown