Neuropeptide Y inhibits vasoactive intestinal peptide‐ and dopamine‐induced cyclic AMP formation in human Ewing's sarcoma WE‐68 cells

FEBS Letters

Jürgens, H.²  Keck, E.¹  van Valen, F.² 

[1] Rheumaklinik II, D-6200 Wiesbaden, FRG;Universitätskinderklinik, Abt. für Hämatologie und Onkologie, D-4000 Düsseldorf, FRG

关键词: Neuropeptide Y;    Pertussis toxin;    cyclic AMP;    (Ewing's sarcoma cell);   

DOI  :  10.1016/0014-5793(89)80639-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neuropeptide Y (NPY) regulation of intracellular cyclic AMP accumulation was studied in human Ewing's sarcoma cell line, WE-68. NPY inhibited vasoactive intestinal peptide (VIP)- and dopamine-stimulated but not basal cyclic AMP formation. The peptide effect was rapid (<2 min), concentration-dependent with a half-maximal effective concentration (EC₅₀) of 8 nM NPY, and maximal inhibition reaching 60–70% with 100 nM NPY. Prior exposure of WE-68 cells to pertussis toxin completely abolished the inhibitory action of NPY. It is concluded that NPY attenuates agonist-stimulated cyclic AMP formation in Ewing's sarcoma WE-68 cells, and may do so via the inhibitory guanine nucleotide regulatory protein of adenylate cyclase.

【 授权许可】

Unknown