Somatostatin and α2‐adrenergic agonists selectively inhibit vasopressin‐induced cyclic AMP accumulation in MDCK cells

FEBS Letters

Friedlander, Gerard¹  Amiel, Claude¹ 

[1] INSERM U.251 and Département de Physiologie, Faculté Xavier Bichat, 16, rue Henri Huchard, F-75018 Paris, France

关键词: (MDCK cell);    cyclic AMP;    Vasopressin;    Somatostatin;    Norepinephrine;    Pertussis toxin;    SRIF;    somatotropin release-inhibiting factor (somatostatin);    MDCK;    Madin-Darby canine kidney;    IBMX;    3-isobutyl-1-methylxanthine;    BSA;    bovine serum albumin;    IAP;    islet-activating protein (pertussis toxin);    HBSS;    Hanks' balanced salt solution;   

DOI  :  10.1016/0014-5793(86)81180-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of somatostatin and α₂-adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in denned medium. These hormones inhibited vasopressin-induced cyclic AMP formation, without affecting either the basal or the glucagon- and prostaglandin E₂-stimulated level. Pretreating the cells with pertussis toxin, or incubating them with MnCl₂ at a low concentration reversed the effect of somatostatin and α₂-agonists. These results suggest that somatostatin and norepinephrine could selectively modulate the renal effect of vasopressin, via the inhibitory regulatory subunit (N_i) of adenylate cyclase.

【 授权许可】

Unknown